Diameter size can be measured by Dynamic Light Scattering (DLS) analysis upon 10x dilution in DPBS.
Yes, if necessary, DIVERSA MRNA DELIVERY NANOPARTICLES can be filtered using small 0.22 µm filters of PES membrane.
If necessary, the DIVERSA MRNA DELIVERY NANOPARTICLES can be concentrated in the buffer exchange step up to desired up to 100uL.
The concentration DIVERSA MRNA DELIVERY NANOPARTICLES depends on the final volume obtained after the buffer exchange step, over 200-250 µL.
Add the volume of your RNA corresponding to encapsulate 5 µg into 900 µL of the Citrate Buffer.
It is recommended to use the RNA at 1 mg/mL. If the concentration of your RNA is lower, you could concentrate it using an Amicon or by SpeedVac.
Yes, if necessary, DIVERSA NANOPARTICLE REAGENT can mix 5 µg RNA (stock: 1 mg/mL) with 5 µg DNA (stock: 1 mg/mL) in a final volume of 900 µL of 10 mM Citrate Buffer pH 3.
You can encapsulate higher amounts. However, the lipid proportion of DIV001 vial must be adapted. For customized prototypes, contact DIVERSA
Yes, DIVERSA MRNA DELIVERY NANOPARTICLES can be loaded with any RNA encoding for your protein of interest. We recommend using this RNA at 1mg/mL.
We have tested in healthy mice to get an insight, but detailed biodistribution should be done with relevant models and with encapsulated drugs / molecules as this affects the biodistribution. We have tried s.c. (subcutaneous) administration using one or more imaging modalities as we have [...]