Your next go-to solution for efficient mRNA delivery

Nanoparticles are particles that measure in nanometers (one billionth of a meter). They exhibit unique properties that differ from those of bulk materials, making them useful in different fields. 

In gene delivery, nanoparticles encapsulate different types of nucleic acids, protecting them from degradation and delivering them to the cells of interest in a safe and efficient fashion.  

There are several methods used to deliver RNA molecules into cells: 

• Mechanical methods, such as electroporation and sonoporation, create temporary pores in cell membranes that enable RNA entry. 
• Chemical methods, such as lipofectamine and PEI, rely on the formation of complexes with cationic lipids or polymers that favor interactions with cell membranes and RNA entry. 
• Viral vectors, such as adeno-associated viruses (AAV), carry RNA molecules and deliver them to target cells. 
• Non-viral delivery systems, such as liposomes, polymeric nanoparticles, and lipid nanoparticles (LNPs), encapsulate, protect, and deliver mRNA to the target cells. The specific properties, safety, and efficacy of transfection depend on the composition of the delivery systems. Among them, LNPs are the only mRNA non-viral delivery systems that have reached the clinic. 

Mechanical and chemical methods typically harm cells and show toxicity, while they cannot be applied in an in vivo setting. Viral delivery, while effective in delivering the genetic material to the nucleus of the cells and helping integrate the DNA into the genome of the host, still poses safety concerns. In this regard, nanoparticles can definitively provide a solution towards the clinical approval of more gene therapies. 

DIVERSA’s nanoparticles are composed of a combination of biodegradable lipids, including one of the main components of cell membranes. This specific combination renders nanoparticles with excellent stability properties and a high rate of cell internalization by different cellular mechanisms. The RNA is efficiently encapsulated and protected from degradation. Additionally, biodistribution can be tailored by modifying the lipid ratios and, if needed, adding specific lipids. 

DIVERSA’s nanoparticles are simple to formulate, without the need for expensive microfluidic equipment and cartridges, and can be prepared before use. They can be acquired with a fluorescent label for cell tracking and monitoring of the cell internalization process. Their transfection properties are superior to more toxic commercial cationic reagents, and they can be used in cells, organoids, and animal models. 

DIVERSA’s nanoparticles can be customized upon request. 

Liposomes and nanoparticles are used in gene delivery, but they differ in structure and properties. 

Liposomes are spherical vesicles composed of one or more phospholipid bilayers surrounding an aqueous core. They are biocompatible and can associate with various polynucleotides, including RNA, typically upon the addition of a cationic lipid to their composition. 

In contrast, DIVERSA’s nanoparticles possess a more organized structure and incorporate key components for efficient encapsulation without relying on cationic lipids. They are designed to optimize intracellular delivery and endosomal escape while minimizing harm to cell membranes. Additionally, DIVERSA’s nanoparticles are patented, signifying a layer of innovation and facilitating clinical translation. 

DIVERSA also offers co-development services  to help you further advance your research project. 

LNPs are a type of carrier used to deliver RNA-based therapeutics, including mRNA vaccines, and have already reached clinical use. 

DIVERSA’s nanoparticles share key similarities with LNPs but boast a simpler composition.  

DIVERSA’s nanoparticles incorporate a cell membrane lipid to enhance intracellular delivery and transfection efficiency, along with a lipid for high stability. Furthermore, commercially available LNPs are offered as complex lipid mixtures requiring microfluidic equipment and cartridges for formulation. This increases formulation costs and necessitates expertise from end-users. In contrast, DIVERSA’s nanoparticles can be readily prepared using a disposable syringe following a simple and rapid protocol. 

Similar to LNPs, DIVERSA’s nanoparticles are scalable and can be formulated under GMP conditions upon request.  

Additionally, DIVERSA offers the possibility of customization for more advanced applications, such as ligand decoration and co-encapsulation of other nucleic acids or biomolecules. 

Based on our experiments so far, we can say that DIVERSA’s Nanoparticles could internalize by a combination of active (endocytosis) and passive (fusion) routes. 

We have tested in healthy mice and models of disease, by subcutaneous, intravenous, and intraperitoneal routes. 

Yes, DIVERSA mRNA DELIVERY NANOPARTICLES can be loaded with any RNA encoding for your protein of interest. We recommend using this RNA at 1mg/mL.  

Yes, if necessary, DIVERSA mRNA DELIVERY NANOPARTICLES can be filtered using small 0.22 µm filters of PES membrane.  

Yes, DIVERSA mRNA DELIVERY NANOPARTICLES can be used in vivo. For specific recommendations and a customized and optimized prototype, contact DIVERSA.