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Tracking intracellular delivery of N3-proteins

DIVTECH FluoGreen Protein Kit, based on reactive lipids, are suitable for click chemistry to conjugate any protein and can be used for tracking and detection of protein loaded nanoemulsions in specific cell lines of interest. The kit uses strongly labeled fluorescent nanoemulsions that are easily internalized by live cells that can be visualized by a wide variety of fluorescent platforms (flow cytometry, microplate assays, fluorescence and confocal microscopy) in less than two hours at Ex/Em = 495/503 nm.

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Technical information
DIV031F1
Flow cytometry analysis of in vitro cellular delivery of DIVTECH FLUOGREEN-PROTEIN in A549 cells after two-hours incubation and compared to untreated cells.
DIV031F1
Intracellular delivery of proteins using DIVTECH FLUOGREEN-PROTEIN kit in A549 cells after two hours incubation. DIVTECH are fluorescently labelled in green (blue: cell nuclei, red: cytoskeleton, green: DIVTECH FLUOGREEN-PROTEIN).

Reference number

DIV031F1

Reagent

DIVTECH FluoGreen Protein Kit

Amount of reagent

1 mL

Molecules delivered

Proteins

Cell types

Adherent & suspension cells, organoids and 3D cultures

Cell culture conditions

Cell culture medium supplemented with/without serum

Number of experiments

1 mL of DIVTECH FluoGreen Broad Range Protein Kit is sufficient to perform 25 delivery experiments in 6-well plates, 69 experiments in 24-well plates and 750 experiments in 96-well plates.

In vivo models

Rodents and zebrafish

Administration route

Intravenous, subcutaneous, intraperitoneal, intra-articular, topical…

Storage

-20 ºC upon arrival, once formulated at 4 ºC

Provided with

1 x DIVTECH empty vial, linker vial, minifilter, 2 tips for 1 mL pipette

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