Enhancing intracellular delivery of a broad range of proteins
DIVTECH Protein Kit, based on reactive lipids, are suitable for click chemistry to conjugate any azide-modified protein. The proteins need to be functionalized with a linker (azide-dPEG8-NHS ester) for an efficient association to DIVTECH by following easy and mild procedures that do not compromise its structure and activity. The azido modified protein will covalently react with the surface of the DIVTECH formulation. The linker for the protein modification is provided within the kit.
Technical information
| Protein | MW (kDa) | pl | DIV031 (BR) |
DIV042 (Anionic) |
|---|---|---|---|---|
Anakinra |
17.3 |
5.5 |
✔ |
✔ |
Green Fluorescent Protein |
28.7 |
~6.0 |
✔ |
|
Luciferase (LucR8) |
37.0 |
8.3 |
✔ |
|
Ovalbumin |
44.5 |
4.6 |
✔ |
✔ |
Integrin ɑ6β4 |
188.8 |
5.5 |
✔ |
✔ |
R-Phycoerythrin |
250.0 |
5.6 |
✔ |
✔ |
β-Galactosidase |
540.0 |
4.6 |
✔ |
✔ |
Examples of proteins efficiently associated with DIVTECH and delivered to cells
Reference number
DIV031
Reagent
DIVTECH Protein Kit
Amount of reagent
1 mL
Molecules delivered
Proteins
Cell types
Adherent & suspension cells, organoids and 3D cultures
Cell culture conditions
Cell culture medium supplemented with/without serum
Number of experiments
1 mL of DIVTECH Broad Range Protein kit is sufficient to perform 225 delivery experiments in 6-well plates, 1150 experiments in 24-well plates and 6815 experiments in 96-well plates.
In vivo models
Rodents and zebrafish
Administration route
Intravenous, subcutaneous, intraperitoneal, intra-articular, topical...
Storage
-20 ºC upon arrival, once formulated at 4 ºC
Provided with
1 x DIVTECH empty vial, linker vial, minifilter, 2 tips for 1 mL pipette
Intracellular delivery of RLuc8 protein to L3.6pl cells using DIVTECH. RLuc8 activity was measured after protein activation and detected by a luminometer. Signal was only detected (455 nm) after using DIVTECH Protein Kit, showing that the protein was efficiently delivered.
