Tracking fluorescent DIVTECH for an effective cell internalization

DIVERSA fluorescently-labelled lipid nanoemulsions for tracking effective cell internalization.

Technical information
DIV000F1
Quantitative flow cytometry analysis of in vitro cellular uptake of DIVTECH FluoGreen Uptake Kit in A549 cells in a time dependent manner (left) and to a large variety of cell lines and primary cells after two-hours incubation (right).

Reference number

DIV000F1

Reagent

DIVERSA FluoGreen cell internalization Reagent

Amount of reagent

1 mL

Molecules delivered

Cell types

Adherent & suspension cells, organoids and 3D cultures

Cell culture conditions

Cell culture medium supplemented with/without serum

Number of experiments

1 mL of DIVTECH Small molecule is sufficient to perform 25 delivery experiments in 6-well plates, 126 experiments in 24-well plates and 750 experiments in 96-well plates.

In vivo models

Rodents and zebrafish

Administration route

Intravenous, subcutaneous, intraperitoneal, intra-articular, topical…

Shipping

Room Temperature

Storage

-20 ºC upon arrival, once formulated at 4 ºC

Provided with

DIVTECH empty vials, 2 tips for 1 mL pipette
DIV000F1
Flow cytometry analysis of in vitro cellular delivery of DIVTECH FluoGreen Uptake Kit in H460 cells after two-hours incubation and compared to untreated cells.
DIV000F1
Confocal microscopy images showing the cell internalization of DIVTECH FluoGreen to fibroblast after two-hours incubation (blue: cell nuclei, red: cytoskeleton, green: DIVTECH FluoGreen).

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