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Tracking fluorescent DIVTECH for an effective cell internalization

DIVTECH FluoGreen Uptake Kit can be used as a positive control for cell internalization before testing the efficiency of associated molecules in specific cell lines of interest, to normalize the values obtained with other types of delivery systems, or for labeling live cells in green fluorescence. DIVTECH FluoGreen Uptake Kit is also a good control for normalizing studies with exosomes. The kit uses strongly labeled fluorescent nanometric emulsions that are easily internalized by live cells that can be visualized by a wide variety of fluorescent platforms (flow cytometry, microplate assays, fluorescence, and confocal microscopy) in less than two hours at Ex/Em = 495/503 nm.

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Technical information
DIV000F1
Quantitative flow cytometry analysis of in vitro cellular uptake of DIVTECH FluoGreen Uptake Kit in A549 cells in a time dependent manner (left) and to a large variety of cell lines and primary cells after two-hours incubation (right).

Reference number

DIV000F1

Reagent

DIVTECH FluoGreen Uptake Kit

Amount of reagent

1 mL

Molecules delivered

Cell types

Adherent & suspension cells, organoids and 3D cultures

Cell culture conditions

Cell culture medium supplemented with/without serum

Number of experiments

1 mL of DIVTECH Small molecule is sufficient to perform 25 delivery experiments in 6-well plates, 126 experiments in 24-well plates and 750 experiments in 96-well plates.

In vivo models

Rodents and zebrafish

Administration route

Intravenous, subcutaneous, intraperitoneal, intra-articular, topical…

Storage

-20 ºC upon arrival, once formulated at 4 ºC

Provided with

DIVTECH empty vials, 2 tips for 1 mL pipette
DIV000F1
Flow cytometry analysis of in vitro cellular delivery of DIVTECH FluoGreen Uptake Kit in H460 cells after two-hours incubation and compared to untreated cells.
DIV000F1
Confocal microscopy images showing the cell internalization of DIVTECH FluoGreen to fibroblast after two-hours incubation (blue: cell nuclei, red: cytoskeleton, green: DIVTECH FluoGreen).

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