Can I use DIVERSA mRNA DELIVERY NANOPARTICLES for in vivo studies?
Yes, DIVERSA mRNA DELIVERY NANOPARTICLES can be used in vivo. For specific recommendations and a customized and optimized prototype, contact DIVERSA.
What’s the difference between LNPs and DIVERSA’s nanoparticles?
LNPs are a type of carrier used to deliver RNA-based therapeutics, including mRNA vaccines, and have already reached clinical use. DIVERSA's nanoparticles share key similarities with LNPs but boast a simpler composition. DIVERSA’s nanoparticles incorporate a cell membrane lipid to enhance intracellular delivery and transfection [...]
What are the main advantages of DIVERSA’s nanoparticles?
DIVERSA's nanoparticles are composed of a combination of biodegradable lipids, including one of the main components of cell membranes. This specific combination renders nanoparticles with excellent stability properties and a high rate of cell internalization by different cellular mechanisms. The RNA is efficiently encapsulated and [...]
What are the advantages of nanoparticles over other transfection methods?
There are several methods used to deliver RNA molecules into cells: Mechanical methods, such as electroporation and sonoporation, create temporary pores in cell membranes that enable RNA entry. Chemical methods, such as lipofectamine and PEI, rely on the formation of complexes with cationic lipids or [...]
Can I filter the formulation?
Yes, if necessary, DIVERSA mRNA DELIVERY NANOPARTICLES can be filtered using small 0.22 µm filters of PES membrane.
Can I use RNA encoding any protein?
Yes, DIVERSA mRNA DELIVERY NANOPARTICLES can be loaded with any RNA encoding for your protein of interest. We recommend using this RNA at 1mg/mL.
Which administration routes have been tried in vivo using this technology?
We have tested in healthy mice and models of disease, by subcutaneous, intravenous, and intraperitoneal routes.
How does the cargo enter the cell?
Based on our experiments so far, we can say that DIVERSA’s Nanoparticles could internalize by a combination of active (endocytosis) and passive (fusion) routes.
What’s the difference between liposomes and DIVERSA’s nanoparticles?
Liposomes and nanoparticles are used in gene delivery, but they differ in structure and properties. Liposomes are spherical vesicles composed of one or more phospholipid bilayers surrounding an aqueous core. They are biocompatible and can associate with various polynucleotides, including RNA, typically upon the addition [...]
How do nanoparticles work in gene delivery?
In gene delivery, nanoparticles encapsulate different types of nucleic acids, protecting them from degradation and delivering them to the cells of interest in a safe and efficient fashion.
